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OXIDASE TEST
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OXIDASE TEST
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Lecture 33: Clinical Biochemistry , Diagnostic enzymes, biomarkers, clinical tests.
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‏📌 Catalase test 🧫 is a biochemical test used to detect the presence of the catalase enzyme in bacteria. This enzyme breaks down hydrogen peroxide (H₂O₂) into water (H₂O) and oxygen (O₂), producing bubbles. 📍Procedure: 1. Place a drop of 3% hydrogen peroxide on a glass slide. 2. Add a bacterial colony using a sterile loop. 📍3. Observe the reaction: Positive ( ): Bubble formation, indicating the presence of catalase. Negative (-): No bubbles, indicating the absence of the enzymes 📍Significan
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